| Alliance | Captain | Round 1 |
|---|---|---|
| Alliance Seed 1 | 10168 LJH Gator Techs | 10284 Team R.O.B.O.T. (Really Obsessed Builders Of Tomorrow) |
| Alliance Seed 2 | 10167 Green Cove Springs Junior High | 9214 Totally Nice Corporation |
| Alliance Seed 3 | 10583 The Robotic Gladiators | 8797 Oakleaf High School |
| Alliance Seed 4 | 13675 Wilkinson Junior High | 10768 Middleburg High School |