| Alliance | Captain | Round 1 |
|---|---|---|
| Alliance Seed 1 | 16468 Green Lemons | 8393 The Giant Diencephalic BrainSTEM Robotics Team |
| Alliance Seed 2 | 11349 VorTech | 14133 Lansdale Catholic Cyber Crusaders |
| Alliance Seed 3 | 16776 MARS: Opportunity! | 20079 MARS: Ingenuity... |
| Alliance Seed 4 | 14464 BotBusters | 22828 Cyclones |